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关键词： 细胞生物   网络教学  

摘要： 细胞生物学是生命科学的四大基础学科之一，知识更新迅速。网络教学突破了传统课堂教学的局限，对构建创新型教学模式有重要意义。针对医学本科专业教学特点，总结建立医学细胞生物学网络教学平台的实践经验；并指出，互动式网络教学有利于知识层面的及时更新，有利于发挥学生的主体作用，有利于加强教师和学生之间的沟通交流。

关键词： 脂肪干细胞  

摘要： 脂肪干细胞（adipose-derived stem cells,ADSCs）是从脂肪组织中分离获得的一种具有自我更新及多向分化潜能的干细胞。2001年,Zuk等[1]通过吸脂术从人体脂肪悬液中首次分离获得,并发现特定条件下可以向肌肉、软骨、脂肪、表皮,造血、神经等多种组织分化。

关键词： 直肠癌   新辅助治疗   生物学特性  

摘要： 目的探讨新辅助放化疗对直肠癌细胞凋亡、增殖以及侵袭能力的影响,为新辅助放化疗在直肠癌中的应用提供依据。方法选取60例新辅助放化疗的中低位直肠癌患者(研究组)及60例未行新辅助放化疗的中低位直肠癌患者(对照组)术后石蜡包埋组织进行对照研究。检测与直肠癌生物学特性相关指标癌胚抗原(CEA)、增殖细胞核抗原(PCNA)及血管内皮生长因子受体2(VEGFR2),比较两组之间的表达差异。结果研究组和对照组CEA在直肠癌组织中的阳性表达分别为83.33%(50/60)、98.33%(59/60),两者比较差异有统计学意义;研究组和对照组PCNA在直肠癌组织中的阳性表达分别为83.33%(50/60)、96.67%(58/60),两者比较差异有统计学意义;研究组和对照组VEGFR2在直肠癌组织中的阳性表达分别为46.67%(28/60)、81.67%(49/60),两者比较差异有统计学意义。结论新辅助放化疗可使直肠癌肿瘤细胞的侵袭能力降低,可能有利于直肠癌患者的预后。

关键词： 黏着斑激酶   白血病   REH细胞   生物学特性  

摘要： 【目的】研究黏着斑激酶(FAK)基因对白血病细胞生物学特性的影响。【方法】构建FAK shRNA慢病毒载体,转染至急性淋巴白血病细胞株REH,同时建立空白载体对照细胞株。通过蛋白质印迹方法检测FAK蛋白表达情况,予荧光染料Annexin V标记细胞观察细胞凋亡情况。体外应用Transwell培养体系,观察白血病细胞在细胞因子SDF-1作用下的迁移能力。将REH细胞予绿色荧光CFSE进行标记,经尾静脉注射到SCID小鼠,观察白血病细胞在动物体内归巢及白血病生成情况。【结果】FAK shRNA慢病毒载体在REH白血病细胞蛋白水平的抑制率为75%。凋亡实验中,空白对照组和FAK基因沉默组Annexin V+细胞百分比分别为(2.19±0.36)%和(6.48±0.58))%。体外迁移实验发现空白对照组与FAK基因沉默组的细胞迁移百分比分别为(50.3±7.22)%和(7.6±3.15)%;动物体内归巢实验发现FAK基因沉默的细胞归巢于骨髓能力明显降低。白血病动物实验发现空白载体对照组小鼠生存时间是40～47 d,而FAK基因沉默组小鼠生存时间是72～80 d。白血病细胞输注后第45天,空白载体对照组与FAK基因沉默组小鼠骨髓中白血病细胞所占百分比分别为(65.5±8.20)%和(9.60±3.65)%。【结论】FAK基因沉默能诱导白血病细胞凋亡,并降低白血病细胞迁移及归巢能力,并抑制白血病生成,提示分子靶向FAK基因有望成为白血病治疗的新策略。

关键词： 细胞生物学实验   综合性   设计性   实验技能   多元化评价  

摘要： 细胞生物学实验教学可以充分发挥学生的主观能动性,培养学生的综合实验技能,进一步加深对细胞生物学基本理论和基本知识的理解。本文从认真做好实验前的准备工作、增加综合性和设计性实验的比例、改进实验课教学方法和教学手段、鼓励学生参与实验课组织教学及实验课考核的多元化评价等方面对细胞生物学实验教学提出了几点建议。

关键词： 胰腺癌   淋巴转移   细胞系  

摘要： 背景与目的:胰腺癌淋巴道转移特性研究越来越受到重视,构建胰腺癌淋巴道转移动物模型能够为相关研究提供理想的实验平台。建立一种具有淋巴道高转移活性的人胰腺癌细胞系,可为深入研究胰腺癌淋巴转移的机理,预防和治疗胰腺癌淋巴转移提供合适的实验平台。方法:通过将人胰腺癌细胞株BxPC-3裸鼠爪垫皮下连续接种法建立淋巴道转移模型,筛选、建立高淋巴转移胰腺癌细胞系,采用流式细胞术检测细胞系中CD133的表达。结果:成功构建了裸鼠人胰腺癌细胞株BxPC-3淋巴道转移模型,获得了具有高侵袭性、高淋巴转移能力的胰腺癌BxPC-3-LN细胞系。该细胞系具有侵袭力强、转移效率高和转移集中的特点,在胰腺原位和爪垫的成瘤能力明显强于母系BxPC-3细胞,其淋巴结转移的发生率明显高于母代细胞。BxPC-3-LN细胞系CD133阳性细胞比例(3%)明显高于母代细胞(0.5%)。结论:该细胞系为胰腺癌转移机理的研究和抗肿瘤药物的筛选提供了合适的实验平台,具有非常重要的科学意义和应用前景。

关键词： 间质干细胞   聚乙烯亚胺   超顺磁性氧化铁   生物学特性  

摘要： 目的探讨应用新型聚乙烯酰胺(PEI)包覆的超顺磁性氧化铁(SPIO)标记骨髓间充质干细胞(bMSCs)对其生物学特性的影响。方法分离、培养贵州小香猪bMSCs,选取第3代bMSCs,用含铁浓度为4、6、8、10、12μg/ml的DMEM/F12培养液孵育24h,未标记组为对照。通过普鲁士蓝染色、透射电镜检查、胎盼蓝染色、MTT检测及成骨、成软骨、成脂诱导分化实验,探讨不同PEI/SPIO浓度对bMSCs标记效率、细胞活性、增殖及分化的影响。结果铁浓度为8μg/ml以上的SPIO标记bMSCs后,普鲁士蓝染色标记效率均接近100%。与对照组相比,Fe浓度为4、6、8μg/ml的PEI/SPIO标记的bMSCs活细胞比率均在95%以上,差异无统计学意义(P>0.05);而Fe浓度为10μg/ml时,活细胞比率约为(80.24±1.34)%,Fe浓度为12μg/ml时,活细胞比率约为(75.44±2.33)%,两者对细胞的活性有明显的抑制作用(P<0.05)。4、6、8g/ml的PEI/SPIO标记的bMSCs成骨、成软骨及成脂分化与正常对照组相比无明显差异,而10、12ug/ml组在诱导培养过程中大部分细胞死亡。结论含铁浓度8μg/ml是PEI/SPIO标记干细胞的适宜浓度,既能高效地标记bMSCs,又不影响bMSCs的细胞活性及增殖分化能力等生物学特性。

关键词： microRNA   kidney   heart   GROWTH-FACTOR-BETA   MATRIX-METALLOPROTEINASE ACTIVITY   CHRONIC LYMPHOCYTIC-LEUKEMIA   MICRORNA-TARGET PAIRS   HEART-FAILURE   DOWN-REGULATION   BROWN-NORWAY   LUNG-CANCER   EXPRESSION   SALT  

摘要： Kriegel AJ, Fang YL, Ding X, Liang M. The miR-29 family: genomics, cell biology, and relevance to renal and cardiovascular injury. Physiol Genomics 44: 237-244, 2012. First published January 3, 2012; doi:10.1152/physiolgenomics.00141.2011.-The human miR-29 family of microRNAs has three mature members, miR-29a, miR-29b, and miR-29c. miR-29s are encoded by two gene clusters. Binding sites for several transcriptional factors have been identified in the promoter regions of miR-29 genes. The miR-29 family members share a common seed region sequence and are predicted to target largely overlapping sets of genes. However, the miR-29 family members exhibit differential regulation in several cases and different subcellular distribution, suggesting their functional relevance may not be identical. miR-29s directly target at least 16 extracellular matrix genes, providing a dramatic example of a single microRNA targeting a large group of functionally related genes. Strong antifibrotic effects of miR-29s have been demonstrated in heart, kidney, and other organs. miR-29s have also been shown to be proapoptotic and involved in the regulation of cell differentiation. It remains to be explored how various cellular effects of miR-29s determine functional relevance of miR-29s to specific diseases and how the miR-29 family members may function cooperatively or separately.

关键词： inflammation   permeability   transport   junctions   endothelium   ENDOTHELIAL BARRIER FUNCTION   HISTAMINE-TYPE MEDIATORS   LIGHT-CHAIN KINASE   PULMONARY-EDEMA   MICROVASCULAR PERMEABILITY   VASCULAR-PERMEABILITY   CAPILLARY-PERMEABILITY   ADENYLYL-CYCLASE   PSEUDOMONAS BACTEREMIA   ALBUMIN PERMEABILITY  

摘要： Ochoa CD, Stevens T. Studies on the cell biology of interendothelial cell gaps. Am J Physiol Lung Cell Mol Physiol 302: L275-L286, 2012. First published September 30, 2011; doi:10.1152/ajplung.00215.2011.-Pain, redness, heat, and swelling are hallmarks of inflammation that were recognized as early as the first century AD. Despite these early observations, the mechanisms responsible for swelling, in particular, remained an enigma for nearly two millennia. Only in the past century have scientists and physicians gained an appreciation for the role that vascular endothelium plays in controlling the exudation that is responsible for swelling. One of these mechanisms is the formation of transient gaps between adjacent endothelial cell borders. Inflammatory mediators act on endothelium to reorganize the cytoskeleton, decrease the strength of proteins that connect cells together, and induce transient gaps between endothelial cells. These gaps form a paracellular route responsible for exudation. The discovery that interendothelial cell gaps are causally linked to exudation began in the 1960s and was accompanied by significant controversy. Today, the role of gap formation in tissue edema is accepted by many, and significant scientific effort is dedicated toward developing therapeutic strategies that will prevent or reverse the endothelial cell gaps that are present during the course of inflammatory illness. Given the importance of this field in endothelial cell biology and inflammatory disease, this focused review catalogs key historical advances that contributed to our modern-day understanding of the cell biology of interendothelial gap formation.

关键词： CD8(+) T-CELLS   L-SELECTIN   TRANSCRIPTIONAL REGULATOR   LINEAGE COMMITMENT   UP-REGULATION   B-CELLS   EXPRESSION   MEMORY   NAIVE   KLF4  

摘要： The Kruppel-like factor family of transcription factors plays an important role in differentiation, function, and homeostasis of many cell types. While their role in lymphocytes is still being determined, it is clear that these factors influence processes as varied as lymphocyte quiescence, trafficking, differentiation, and function. This review will present an overview of how these factors operate and coordinate with each other in lymphocyte regulation. The Journal of Immunology, 2012, 188: 521-526.