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关键词： 大科学   延缓衰老   衰老细胞   杂志   美国   细胞生物学   疾病   清除  

摘要： 美国（（Science））杂志评出2011年十大科学突破。本刊连续给予了介绍。本刊前两期（中国细胞生物学学报2012；34（2）：195．6．中国细胞生物学学报2012；34（3）：295-7．）分别介绍了在阻断艾滋病传播和光合作用研究领域所取得的突破性研究成果，本期重点介绍科学家们在抗衰老领域所取得的研究成果。

关键词： 干细胞生物学   血液学   征文通知   博士生   北京协和医学院   论坛   博士研究生   学术交流  

摘要： 为推动我国血液学和干细胞生物学的发展，为优秀博士研究生提供一个高起点、跨领域的学术交流平台，促进优秀知识成果的展示和传播，由北京协和医学院主办，

关键词： 纳米传感器   实时监测   创新技术   相互作用   细胞间   美国科学家   细胞生物学   生长情况  

摘要： 美国科学家研发出了一种新技术,将纳米传感器＂贴＂在细胞膜表面,可实时监测细胞间的相互作用,清晰度远超以往。这项创新技术能让科学家进一步理解复杂的细胞生物学,监测移植细胞的生长情况以及为疾病研发出有效的治疗方法。最新研究发表在今年7月出版的《自然.纳米技术》杂志上。

关键词： 细胞生物学   教学方法   PBL教学  

摘要： 为了加深医学生对细胞生物学与医学之间关系的理解,同时适应现代社会对医学生综合素质培养的要求,在医学细胞生物学教学中引入了PBL教学法。实践证实,PBL教学模式能够提高学生的综合素质和教师的业务水平,值得倡导和推广。

关键词： 细胞生物学   哈佛大学   国际   中国   生命活动规律   生命科学   转化医学   机体结构  

摘要： 细胞是机体结构和功能的基本单位，细胞生物学以细胞为研究对象，揭示细胞的增殖、分化、遗传、运动、衰老等基本生命活动规律，是生命科学的基石。 细胞生物学是生命科学的基础学科，与医学、药学以及生物学其他学科广泛交叉与融合，成为生命科学的交汇点，并外延至重大疾病的转化医学。

关键词： genetic modification   swine   zinc finger nuclease   PIGS   KNOCKOUT   CLEAVAGE   MOUSE   MICROINJECTION   MUTAGENESIS   DROSOPHILA   TARGETER   MODEL   ZIFIT  

摘要： Engineered zinc finger nucleases (ZFN) are rapidly gaining popularity as a means to enhance the rate and specificity of DNA modifications in plant and animal cells. Repair-mediated gene modification by ZFN is driven by introducing DNA double-strand breaks via a nonspecific nuclease domain linked to a sequence-specific zinc finger nucleotide recognition domain. This review examines the use of ZFN to produce genetically modified swine and the potential of this technology for the future. By combining conventional gene targeting methods with somatic cell nuclear transfer, several genetically modified pig models have been produced. These conventional techniques are inefficient in mammalian somatic cells and provide little control over the site specificity and rate of exogenous DNA integration. The use of engineered ZFN that bind and cleave genomic DNA at specific loci can enhance targeting efficiencies by orders of magnitude. Recent publication of the first genetic modification in pigs by combining ZFN technology with somatic cell nuclear transfer has opened the door to genome targeting with a precision that was not previously possible in a large animal model. Since that time, model pigs with selective knockout of endogenous genes have been produced. This review will examine the use of ZFN to generate these pig models and the potential of ZFN to accelerate the production of genetically modified pigs of agricultural and biomedical importance. Current methods of ZFN design, important considerations for their safe and effective use in modification of the swine genome, and future innovative applications of this technology in pigs will be discussed.

关键词： CYTOLOGY -- Congresses   CONFERENCES & conventions  

摘要： The article focuses on the topics discussed at the Cell Biology Symposium titled "Novel technologies and novel insights," held in New Orleans, Louisiana, from July 10-14, 2011. One of the speakers of the symposium, Jeffrey Whyte of the University of Missouri, spoke about the conventional and emerging technologies for the modification of genome in mammals, like swine. Another speaker was Rebecca Wattam of Virginia Polytechnic Institute.

关键词： p27   CREB   inflammation   CCL4   MMP9   P27(KIP1) PHOSPHORYLATION   CELL   CHEMOKINE   DIFFERENTIATION   GRANULOCYTES   INFLAMMATION   ASSOCIATION   MECHANISM   PATHWAY   T198  

摘要： Neutrophils are emerging as important mediators in cancer progression. Recent studies associated neutrophils with poor clinical outcome of HNC patients and showed that HNC induces recruitment, survival, and release of proinflammatory factors by neutrophils in vitro. The molecular mechanisms through which HNC and other cancers modulate neutrophil biology are currently unknown. To explore these mechanisms, we used an in vitro system that models the interaction between human HNC cells and neutrophils or neutrophilic-differentiated HL-60 cells, respectively. We show that HNC-derived factors activate p38-MAPK in neutrophils, which partly promotes neutrophil survival, but not neutrophil recruitment and motility. Most importantly, HNC-induced p38-MAPK activation strongly stimulates the release of CCL4, CXCL8, and MMP9 by neutrophils. We identify CREB and interestingly, p27 phosphorylated at T198 as downstream members of the HNC-induced p38-MAPK signaling cascade. Using siRNA technology, we demonstrate that p27 and CREB mediate the release of CCL4 and CXCL8 and that CREB, additionally, mediates the release of MMP9. These data unravel novel molecular mechanisms involved in regulation of neutrophil proinflammatory functions. Our studies on human HNC tissues indicate that tumor-infiltrating neutrophils might be a major source of CCL4 and particularly, MMP9 in cancer patients. Thus, our findings provide novel, mechanistic insights relevant for the pathophysiology of HNC and possibly, other types of cancer as well. J. Leukoc. Biol. 91: 591-598; 2012.

关键词： PLANARIAN STEM-CELLS   ZEBRAFISH HEART REGENERATION   SALAMANDER LIMB REGENERATION   APOPTOTIC CELLS   ANIMAL REGENERATION   TISSUE REGENERATION   SYSTEM REGENERATION   TAIL REGENERATION   NERVOUS-SYSTEM   IMAGINAL DISCS  

摘要： Regeneration of complex structures after injury requires dramatic changes in cellular behavior. Regenerating tissues initiate a program that includes diverse processes such as wound healing, cell death, dedifferentiation, and stem (or progenitor) cell proliferation; furthermore, newly regenerated tissues must integrate polarity and positional identity cues with preexisting body structures. Gene knockdown approaches and transgenesis-based lineage and functional analyses have been instrumental in deciphering various aspects of regenerative processes in diverse animal models for studying regeneration.

关键词： 菌物学   征稿简则   学报   中国科学院   系统分类学   资源开发利用   细胞生物学   区系地理学  

摘要： 1刊物简介 《菌物学报》（曾用名《真菌学报》、《菌物系统》）1982年创刊，是中国科学院主管的菌物学学术期刊，报道菌物（真菌、粘菌、卵菌等）学研究领域最新成果。主要刊登菌物系统分类学、菌物分子与细胞生物学、菌物区系地理学、菌物多样性及资源开发利用、动植物病原菌物学、医学真菌学、食用和药用真菌学等方面的研究论文、简报和综述。本刊是中国自然科学核心期刊，也是国际菌物学界信赖的刊物，已被国内外多家权威检索系统收录。