关键词： Mycobacterium bovis   PGL   PDIM   diagnosis-bacteria   TUBERCULOSIS   IDENTIFICATION   EPIDEMIOLOGY   DIAGNOSIS  

摘要： Sixteen Mycobacterium bovis (M. bovis) strains isolated from bovine tissues and one standard reference strain of M. bovis AN5 alongwith other species of mycobacteria for comparison were investigated for the presence of phenolic glycolipid (PGL) and phthiocerol dimycocerosate (PDIM) for rapid identification of M. bovis by thin-layer chromatography (TLC). The study indicated presence of PGL with an Rf value of 0.75 in chloroform-methanol solvent in all 17 M. bovis strains. The dimycocerostate A corresponding to spot A was the major constituent among all the three spots in M. bovis strains. TLC appeared to be a promising alternative to conventional biochemical methods for identification of M. bovis taking into consideration bath PGL and PDIM lipids. (C) 1999 Elsevier Science B.V. All rights reserved.

关键词： 调压器   多功能集成   涡轮式   调压计量站   涡轮流量计   调压站   止回阀   解决方法   固有特性   研究中心  

摘要： 调压计量站的结构,至今已做过多种尝试,原因是必须综合考虑技术、经济、规章制度和环保方面的种种限制,从而使它成为一个非常紧凑、但不影响其组成的固有特性的装置。PDIM 涡轮式多功能集成调压器,是法国煤气公司研究中心与一家法国企业 CD GAZ 密切合作的成果,是一种具有开创性的解决方法。

关键词： corrosion   iron, Fe  

摘要： It is demonstrated that PDIM imaging allows the observation of localized electrochemical reactions with subnanoscopic vertical resolution.

关键词： Phthiocerol dimycocerosate   PDIM   Phenolic glycolipid   PGL-I   Phosphatidyl inositol mannoside   PIM   Mycolates   Leprosy skin biopsies  

摘要： Lipids were extracted from small skin biopsies obtained from 5 lepromatous leprosy patients by employing a procedure designed to produce separate non-polar and polar fractions. The defatted tissue was subjected to alkaline hydrolysis and the total fatty acids in the hydrolysate were converted to methyl esters by a phase transfer reaction using iodomethane. The composition of free lipids and methyl esters of fatty acids was analysed by special thin-layer chromatographic systems. The presence of dimycocerosates of phthiocerol A, phthiocerol B and phthiodiolone A, phenolic glycolipid-I, two phosphatidyl inositol mannosides and 2 mycolates − alpha- and keto-mycolate − was demonstrated using reference lipid samples.